Purpose: Elevated body iron stores could promote oxidative stress, and in this manner affects the pathogenesis of insulin resistance. In this study, we investigated the relationship between body iron stores represented in serum ferritin and transferrin saturation together with insulin resistance and other cardio metabolic risk factors. Material and methods: In Egyptian subjects, we measured fasting serum ferritin and Transferrin saturation (TR %) was calculated with the formula: (Serum iron level X 100) / total iron-binding capacity in one hundred sixty five Egyptian subjects divided as controls, obese and obese diabetics. Body mass index (BMI), waist circumference (WC) and blood pressure were measured in all subjects. Insulin resistance was assessed using the homeostasis model assessment for insulin resistance (HOMA-IR). All subjects were investigated to analyse the change in their total cholesterol, HDL-C, non-HDL-C, and triglycerides levels. Results: Serum ferritin was significantly higher (p<0.05) in diabetic compared with non-diabetic subjects, serum ferritin also higher in obese non diabetics compared to non-obese controls. However, this difference did not reach the level of significance. There was non-significant difference in transferrin saturation (TR %) among studied groups. Significant positive correlation was seen between serum ferritin & BMI, WC and diastolic blood pressure, significant negative correlation between it & HDL-C and non-significant positive correlation with systolic blood pressure, fasting blood glucose, Hb A1C and non-HDL-C. Conclusion: serum ferritin associated with diabetes mellitus and cardio metabolic risk factors, while transferrin saturation did not.