The present work describes development and validation of a new simple, accurate, precise and selective stability- indicating high performance thin layer chromatographic (HPTLC) method for simultaneous estimation of Ilaprazole and Domperidone in capsule dosage form as per ICH guidelines. Separation of both drugs was performed by using precoated with silica gel 60 F254aluminium plates (10 ×10 cm) with 250 µm thickness as stationary phase and mixture of Ethyl acetate: Methanol: Ammonia: Triethylamine (8:1: 0.5: 0.5, v/v/v/v) as a mobile phase. Densitometric scanning was carried out at 297 nm. Ilaprazole and Domperidone were satisfactorily resolved with retention factor values of 0.30±0.05 and 0.68±0.03, respectively. Linear responses were observed in the concentration range of 100-600 ng band-1 and 300-1800 ng band-1for Ilaprazole and Domperidone, respectively. The % assay (Mean ± S.D.) was found to be 100.33±1.23 for Ilaprazole and 100.41± 0.77 for Domperidone. Intra-day, as RSD (%), was found to be in the range of 0.52 to 0.89 for Ilaprazole and 0.22 to 0.28 for Domperidone. Inter-day variation, as RSD (%) was found to be in the range of 0.14 to 0.60 for Ilaprazole and 0.17 to 0.23 for Domperidone. The developed method can be used for the analysis of Ilaprazole and Domperidone without any interference from the excipients and can be successfully used to estimate the amount of each drug in the formulations by easily available low cost materials.
Swedana (fomentation) is defined as the process by which the sweat or perspiration is produced in the body by using various methods. Inducing perspiration by using heated round Pinda (bolus) of special rice i.e, Sashtika Shali prepared in milk processed with herbal decoction is referred as Shashtikashali Pinda Sweda. Therapeutic Swedana is a process of heating by which the body is induced to produce sweat to mitigate several local and systemic disorders. Swedana helps in producing unparallel relief in pain, stiffness and heaviness in body associated with many systemic diseases. Shashtikashali Pinda Swedena is included under the catogery of Pinda Swedana (Bolus formation). It is also well known as Navarakhizi. This belongs to the category of Agni sweda as well as Snigdha Sweda. A Pinda (bolus) made up of Shashtikashali (a type of rice harvested in 60 days) prepared in milk processed with herbal decoction is tied in bolus to rub against the whole body or afflicted part of the body so as to provide heat, nutrition and strength to the pain afflicted joints, muscles or body parts. This is an easy and effective process of Swedana and is an ideal in healthy persons, children and in illness due to morbid Vata Dosha like diseases of nervous system, spinal cord degeneration, neuropathy, chronic rheumatism, backache, Mamsagata Vata (Myalgia), Fibromyalgia, Lumbar spondylosis, Cerebral palsy, Muscular dystrophy. Shashtikashali Pinda Sweda works as, Swedana, Snehana and Brimhana altogether and is widely used in Vata Vyadhi.
Dye degrading microorganisms’ viz., Pseudomonas sp, Bacillus sp. was isolated from dye effluent soil sample. The isolated colonies were identified by morphological, cultural and biochemical characterization. Different concentrations (10, 50 and 100µg) of dyes such as navy blue, acid brown and pen BRL were degraded by Pseudomonas sp, and Bacillus sp. respectively. Efficiency of dye degradation by these two organisms was detected by UV spectrophotometer. Out of two dyes at different concentrations acid brown (100µg) was effectively degraded by Bacillus sp. And Navy blue was degraded by Pseudomonas sp. The current research highlights the widespread occurrence of dye degrading microorganisms in the Indian environment, where human activities like leather/textile industries are common. Based on the present findings, it may be summarized that the enzymes viz., azoreductase and oxidative enzymes like lignin peroxidase, laccases have enormous potential applications in degradation of dye in leather/textile industries