To study the phytochemical screening and anticancer activity of methanolic extract from Eucalyptus globulus leaves of medicinal plants. The different types of phytochemical analysis tested by using standard procedure and anticancer activity of methanolic extract using on Hela cancer cell line evaluated by MTT assay. In the present investigation, the preliminary phytochemical analysis of these plants confirm the occurrence of diverse range of phytochemicals like flavonoids, phenols, tannins, terpenoids, catechins and steroids. The occurrence of result revealed Eucalyptus globulus found significant cell inhibiting activity against HeLa cancer cell line and IC50 value was 43.6423µg/ml.
Lead (Pb), unlike some other heavy metals, is not essential for higher plants and other organisms. Even at moderate concentrations, lead is highly toxic to man, animals and plants that is why it is considered a dangerous pollutant. In order to quantitatively decipher the effect of the concentrations of lead on the germination and growth of seedling of black gram (Vigna mungo), seed germination parameters were observed using fresh seeds treated with various concentrations of lead nitrate. The study was planned to delve into understanding the possible toxicity effects on seeds upon exposure to lead pollution. Seeds of this plant were exposed to four different concentrations of the metal (e.g. 0.001%, 0.01%, 0.1%, and 1%, w/v). From the results of germination experiments, a decrease in the seed germination parameters with concomitant increase in the heavy metal concentration was observed, and the inhibitions of seedling growth were clearly noticeable above certain acute concentrations (e.g. 1% of lead ion concentration). The results of the study revealed that lead unfavorably influenced the growth parameters as well as biochemical elements of seedling on increasing the metal ion concentration.
The anoxygenic photosynthetic purple non sulphur bacteria are versatile in their habitats and were reported to degrade and remove toxic chemicals in polluted waters. In the present study, 5 photosynthetic purple non sulphur bacteria were isolated from fresh water, Rice fields and Effluents of boiled rice industry from different places of Mahabubnagar and surrounding areas. The isolates were named as KSL-1, KSG-1, BKL-2, KRM-3 and BKG-2 based on the type of the sample, place of isolation and the carbon source utilized. Out of these, KSL-1, KSG-1 and BKL-2 independently, and a mixture of these 3 isolates were used for bioremediation of Agricultural runoff and Domestic sewage. Physical parameters like temperature and pH of the wastes were recorded which varied from 290C - 390C and 6.0 - 8.0 respectively in untreated and treated samples. Chemical parameters like BOD, COD, TDS, TSS, chlorides and sulphates were estimated in untreated samples and samples incubated with the bacteria for 3, 5, 7 and 9 days of incubation. Treatment of Agricultural runoff with these bacteria resulted in overall reduction of BOD 27.4 – 33.5%, COD 13.13 – 46.92%, TDS 16.67 - 50%, TSS 12.5 - 75%, chlorides 16.43 – 46.61% and sulphates 5.47 – 11.64% by 9th day of incubation. Similarly, treatment of Domestic sewage with these bacteria resulted in reduction of BOD 21.1 - 60.2%, COD 16.69 – 63.89%, TDS 33.33 - 48.33%, TSS 47.4 – 80.25%, chlorides 19.34 – 39.62% and sulphates 2.93 – 17.11%. A combination of the three cultures was more effective in reducing the concentration of wastes.
Simple, sensitive and selective methods are developed for the indirect and oxidative spectrophotometric determination of drugs, viz., Phenylephrine Hydrochloride, Ramipril Hydrochloride, Sumatriptan Succinate, Tramadol hydrochloride and Trimetazidine is based on their reactivity towards Chloramine-T (ChL-T). The method is based on the oxidation of drugs by ChL-T (Excess quantity) and estimating the amount of un-reacted ChL-T by Safranin-O dye at λmax 524 nm. The concentration reacted Chloramine-T is equal to the Drug concentration in this method. The concentration of Safranin-O and Chloramine-T is 10 µg mL-1. The calibration curves obeyed Beer’s law over the concentration range of 10-70 µg mL-1 (PHP), 5-35 µg mL-1 (RAM), 5-35 µg mL-1 (SUM), 10-70 µg mL-1 (TRA) & 12-84 µg mL-1 (TMT). This method has been applied for the determination of drugs in their pure form as well as in tablet formulations. The method has been validated in terms of guidelines of International Conference on Harmonization.
Abies pindrow is a tall evergreen tree with a conical crown, attaining a height up to 75 m and girth of 7 m, found in temperate Himalayas from Kashmir to Nepal at altitude of 2100-3600 m. Plant is reported to possess several medicinally important chemical constituents viz lactones, chalcones, fatty acid, hydrocarbons etc. Plant is reported to possess medicinal activities like anti-microbial, anti-inflammatory, anxiolytic etc. The present study pertains to Pharmacognostical, physiochemical and toxicological investigation of this medicinally important plant was conducted to establish a pharmacognostic standard of the bark of Abies pindrow. The study has been conducted according to guidelines of World Health Organization and Ayurvedic Pharmacopoeia of India. Water, alcohol, chloroform, petroleum ether soluble extractive value was found to be 3.75%w/w, 21.5%w/w, 1.5%w/w, 0.9%w/w respectively by hot method. Total ash, acid insoluble ash, water soluble ash, sulphate soluble ash was found to be 1.5%w/w, 0.1w/w, 0.6w/w, 0.13w/w respectively. Loss on drying, swelling index, fibre content, foaming index, bitterness value, tannin content was found to be 6.37%w/w, 1cm, 2.3, 0.6ml/mg, 2000, 1.66%w/w respectively . Content of heavy metal and microbial count was found within the acceptable range. Preliminary phytochemical screening revealed the presence of carbohydrates, phenolic compounds, protein and free amino acids, saponins, sterols, tannins and flavonoids
An improved protocol for the synthesis of benzimidazole derivatives, starting from ethyl 3-iodobenzoate is reported. In this present communication we report synthesis of 3-(1H-benzo[d]imidazol-1-yl)phenyl aryl amide derivatives in good yields. All title compounds were characterized by IR, NMR and Mass spectral analyses